Phenolyog ib qho molecule uas ua lub luag haujlwm tseem ceeb hauv ntau cov tshuaj tiv thaiv thiab siv dav hauv kev lag luam. Yog li ntawd, nws yog ib qho tseem ceeb kom muaj ib txoj kev txhim khu kev qha los txheeb xyuas phenol hauv ntau cov qauv. Hauv tsab xov xwm no, peb yuav tshawb txog ntau yam txuj ci muaj los txheeb xyuas phenol, lawv qhov zoo thiab qhov tsis zoo, thiab qhov tseem ceeb ntawm kev txheeb xyuas phenol hauv lub neej niaj hnub thiab kev lag luam.

1. Gas Chromatography (GC)

Gas chromatography yog ib txoj kev siv dav siv los txheeb xyuas cov phenol. Nyob rau hauv txoj kev no, tus qauv yog txhaj rau hauv ib kem uas muaj cov theem nyob ruaj khov. Cov theem mobile ces ntws los ntawm kem, cais tus kheej cov khoom ntawm tus qauv. Kev sib cais yog nyob ntawm qhov txheeb ze solubility ntawm cov khoom hauv cov theem nyob ruaj ruaj thiab mobile.

Qhov zoo: GC yog qhov rhiab heev, tshwj xeeb, thiab nrawm. Nws tuaj yeem kuaj pom qhov qis qis ntawm phenol.

Qhov tsis zoo: GC xav tau cov neeg ua haujlwm tau txais kev cob qhia thiab cov khoom kim heev, ua rau nws tsis tsim nyog rau kev xeem.

2. Liquid Chromatography (LC)

Cov kua chromatography zoo ib yam li cov roj chromatography, tab sis cov theem nyob ruaj khov tau ntim rau hauv ib kem es tsis txhob coated rau ntawm qhov chaw ruaj khov. LC feem ntau yog siv los cais cov molecules loj, xws li cov proteins thiab peptides.

Qhov zoo: LC muaj kev sib cais ua haujlwm siab thiab tuaj yeem tuav cov khoom loj.

Qhov tsis zoo: LC tsis tshua muaj siab dua GC thiab xav tau sijhawm ntxiv kom tau txais txiaj ntsig.

3. Spectroscopy

Spectroscopy yog ib txoj kev tsis muaj kev puas tsuaj uas suav nrog kev ntsuas qhov nqus lossis emission ntawm hluav taws xob los ntawm atom lossis molecules. Nyob rau hauv rooj plaub ntawm phenol, infrared spectroscopy thiab nuclear magnetic resonance (NMR) spectroscopy feem ntau yog siv. Infrared spectroscopy ntsuas qhov nqus ntawm infrared hluav taws xob los ntawm cov molecules, thaum NMR spectroscopy ntsuas qhov nqus ntawm radiofrequency hluav taws xob los ntawm nuclei ntawm atoms.

Qhov zoo: Spectroscopy yog qhov tshwj xeeb heev thiab tuaj yeem muab cov ncauj lus kom ntxaws txog cov qauv ntawm cov molecules.

Qhov tsis zoo: Spectroscopy feem ntau xav tau cov cuab yeej kim thiab tuaj yeem siv sijhawm ntev.

4. Txoj kev Colorimetric

Colorimetric txoj kev koom nrog reacting ib tug qauv nrog ib tug reagent los tsim ib cov khoom xim uas yuav ntsuas spectrophotometrically. Ib txoj kev colorimetric ntau rau kev txheeb xyuas phenol cuam tshuam nrog cov qauv nrog 4-aminoantipyrine nyob rau hauv lub xub ntiag ntawm ib co coupling reagent los tsim cov khoom liab-xim. Qhov kev siv ntawm cov xim yog ncaj qha proportional rau lub concentration ntawm phenol nyob rau hauv cov qauv.

Qhov zoo: Colorimetric txoj kev yog qhov yooj yim, pheej yig, thiab tuaj yeem siv rau kev sim teb.

Qhov tsis zoo: Cov txheej txheem Colorimetric yuav tsis muaj qhov tshwj xeeb thiab yuav tsis pom txhua hom phenol.

5. Kev soj ntsuam lom neeg

Kev soj ntsuam lom neeg siv cov tshuaj tiv thaiv kab mob tshwj xeeb ntawm cov kab mob txhawm rau txheeb xyuas qhov muaj, cov khoom, thiab cov ntsiab lus ntawm cov hom phiaj. Piv txwv li, qee cov kab mob thiab cov poov xab tuaj yeem hloov phenol rau cov khoom xim uas tuaj yeem ntsuas spectrophotometrically. Cov kev ntsuam xyuas no yog qhov tshwj xeeb heev tab sis yuav tsis muaj rhiab heev ntawm qhov tsis tshua muaj siab.

Qhov zoo: Cov tshuaj lom neeg lom neeg muaj qhov tshwj xeeb thiab tuaj yeem siv los txheeb xyuas cov tebchaw tshiab.

Qhov tsis zoo: Kev soj ntsuam lom neeg muaj peev xwm tsis muaj kev nkag siab zoo thiab feem ntau siv sijhawm.